MARCH5 Antibody #19168

Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape that regulate metabolism and cell death, and have significant consequences on health and disease (1,2). These processes are largely controlled by mitochondrial dynamin-related GTPases and associated proteins, including mitofusin-1 (MFN1), mitofusin-2 (MFN2), OPA1, MFF, Fis1, and DRP1. DRP1, MFF, and Fis1 regulate mitochondrial fission, while the mitofusins and OPA1 control fusion.
MARCH5/MARCH-V/MITOL is a ubiquitin E3 ligase localized to the mitochondrial outer membrane that affects mitochondrial dynamics (3-5). MARCH5 has been associated with tumorigenesis, innate immunity, autophagy, apoptosis, and senescence (3-9). Mechanistically, MARCH5 associates with MFN2 and also promotes the ubiquitination of fission proteins DRP1, as well as hFis1 and MFN1 (3-6). Dysregulation of MARCH5 through overexpression, mutation, or knockdown can regulate the turnover of fission and fusion proteins and lead to changes in mitochondrial dynamics (3-6). Studies have shown that elevated levels of MARCH5 in ovarian and breast cancers potentially contribute to tumor growth and metastasis (10,11).