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ETS-1 (D8O8A) Rabbit Monoclonal Antibody

ETS-1 (D8O8A) Rabbit Monoclonal Antibody #14069

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	52
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence
ChIP-Chromatin Immunoprecipitation
C&R-CUT & RUN
C&T-CUT & Tag

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652.

The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

Application	Dilution
Western Blotting	1:2000
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:3200 - 1:6400
Chromatin IP	1:50
Chromatin IP-seq	1:50
CUT&RUN	1:50
CUT&Tag	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #98536.

实验步骤

Specificity / Sensitivity

ETS-1 (D8O8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETS-1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein.

Background

ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9).

Alternate Names

Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; c-ets-1; ets protein; ETS proto-oncogene 1, transcription factor; ETS-1; ETS1; EWSR2; FLJ10768; p54; Protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)

Database Links

UniProt ID: P14921

Entrez-Gene Id: 2113

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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