为什么我的单细胞数据中有大量双联体/多联体？

添加甲醇固定缓冲液的速率会影响细胞聚集。In Cell Fixation (Day 1) - step 7 of the InTraSeq protocol, it is very important to dispense the methanol over a period of at least 60 seconds. 如果不这样做，可能会产生双重/多重的结果。

If cell clumps are still observed after Washes and Count (Day 3) - step 8 of the InTraSeq protocol, additional pipette mixing is recommended before proceeding to the single-cell experiment.

Last updated: 2024 年 9 月 18 日