Which secondary antibodies are compatible with your chimeric antibodies?

We have vetted secondary antibodies from multiple vendors and recommend the following:

Anti-Rabbit Fc
Rockland Immunochemicals	611-1223	Rabbit IgG Fc Antibody Fluorescein Conjugated Pre-Adsorbed
ThermoFisher Scientific	A78954	Goat anti-Rabbit IgG Fc, Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555
Jackson ImmunoResearch	111-585-046	Alexa Fluor® 594 AffiniPure™ Goat Anti-Rabbit IgG, Fc fragment specific
Rockland Immunochemicals	611-1923	Rabbit IgG Fc Antibody Texas Red™ Conjugated Pre-Adsorbed
ThermoFisher Scientific	A78957	Goat anti-Rabbit IgG Fc, Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647
Anti-Cat
Rockland Immunochemicals	602-1203	Cat IgG Fc Antibody Fluorescein Conjugated
Rockland Immunochemicals	602-1903	Cat IgG Fc Antibody Texas Red™ Conjugated
Jackson ImmunoResearch	102-605-003	Alexa Fluor® 647 AffiniPure™ Goat Anti-Cat IgG (H+L)
Anti-Horse
Rockland Immunochemicals	608-1003	Horse IgG Fc Antibody Rhodamine Conjugated
Rockland Immunochemicals	608-1903	Horse IgG Fc Antibody Texas Red™ Conjugated
Anti-Mouse*
Cell Signaling Technology	94207	Goat Anti-Mouse IgG2a Antibody (Alexa Fluor® 488 Conjugate)
Cell Signaling Technology	51761	Goat Anti-Mouse IgG2a Antibody (Alexa Fluor® 555 Conjugate)
Jackson ImmunoResearch	115-585-206	Alexa Fluor® 594 AffiniPure® Goat Anti-Mouse IgG, Fcγ subclass 2a specific
Cell Signaling Technology	30135	Goat Anti-Mouse IgG2a Antibody (Alexa Fluor® 647 Conjugate)

*Pan anti-mouse secondary antibodies (4408, 4409, 8890, 4410) successfully detect mouse IgG2a chimeric primary antibodies, however it is suggested that users determine the optimal dilution for each primary antibody by titration. Pan anti-mouse secondary antibodies must be titrated past 1:2000 to avoid cross-reactivity with other chimeric primary antibodies when multiplexing.

Last updated: 2025 年 7 月 25 日

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