violetFluor 450 Antibody Conjugates

Maximize multiplexing potential in flow cytometry and immunofluorescence workflows with photostable violetFluor 450. Excited by the 405 nm violet laser line, this blue dye has increased water solubility and labeling efficiency and offers reduced background fluorescence.

Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with CD8α (2.43) Rat mAb (violetFluor 450 Conjugate) #17083 (left, blue) and colabeled with CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (right, green) and DRAQ5 #4084 (right, red).

View violetFluor 450 Conjugated Antibodies

属性

激发：405 nm	发射波长：450 nm
激光线：405 nm	常用滤光片组：450/50

The dashed blue line shows the excitation spectrum of violetFluor 450, while the solid line represents its emission spectrum. When selecting compatible antibodies for multiplexing experiments, it is important to avoid emission overlap between antibodies. Optimally excited by the 405 nm violet laser line, violetFluor 450 has an excitation peak quite distinct from its emission peak.

研究应用

violetFluor 450 conjugates offer several key benefits in scientific research:

violetFluor 450 is ideal for high-parameter flow cytometry, compatible with violet lasers, it can be differentiated from dyes with longer emission wavelengths.
Due to its low background and photostability, violetFluor 450 is advantageous for the detection of fluorescently labeled molecules at low concentrations.

The stability of violetFluor 450 conjugates allows them to be utilized in a broad range of experimental applications:

Reveal Cell Localization with High-Precision

Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with CD4 (RM4-5) Rat mAb (violetFluor 450 Conjugate) #92599 (blue, left) and colabeled with CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399 (green, right) and Iba1/AIF-1 (E4O4W) XP^® Rabbit mAb (Alexa Fluor^® 555 Conjugate) #36618 (red, right).

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use violetFluor 450 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. 对于定制或定制化偶联物，我们凭借数十年的经验、全面的生物物理表征和亲本抗体验证来提供具有高特异性和敏感性的产品。每个订单都附有全面的产品文档，并可享专家技术支持。

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Explore Our violetFluor 450 Conjugated Antibodies


CD3 (UCHT1) Mouse mAb (violetFluor 450 Conjugate) #61347	F4/80 (BM8.1) Rat mAb (violetFluor 450 Conjugate) #40781	CD44 (IM7) Rat mAb (violetFluor 450 Conjugate) #53289