PerCP-Cy®5.5 Antibody Conjugates

Leverage the tandem fluorophore PerCP-Cy^®5.5 for improved photostability and brightness over PerCP alone. Due to the resonance energy transfer from PerCP to Cy5.5, you can enhance the utility of your 488 laser line, making bright PerCP-Cy^®5.5 conjugated antibodies an excellent choice for multiplexed flow cytometry workflows and assays targeting less abundant antigens.

Flow cytometric analysis of live mouse splenocytes using NK1.1 (CD161) (PK136) Mouse mAb (PerCP-Cy ^® 5.5 Conjugate) #88957 (right) compared to concentration-matched Mouse Isotype Control (PerCP-Cy ^® 5.5 Conjugate) #24589 (left).

View PerCP-Cy^®5.5 Conjugated Antibodies

属性

PerCP-Cy^®5.5 conjugated antibodies have a far-red emission spectrum (solid red) with reduced emission overlap with many commonly used fluorophores. With an excitation maximum of 482 nm (dashed red line), the same laser line can be used with other fluorophores, such as FITC or PE, for easy integration into multicolor panels.

研究应用

Several properties of PerCP-Cy^®5.5 make it a powerful research tool, such as:

• Brighter than PerCP alone due to improved energy transfer. This makes it ideal for detecting low-abundance markers or applications requiring a strong signal strength with limited autofluorescence.
• Excitable by the standard 488 nm laser, it is a convenient choice, as no additional lasers or modifications to equipment are required.
• Causes less Fc receptor-mediated nonspecific staining than other standard tandem fluorophore conjugates.

Resistance to photobleaching makes PerCP-Cy^®5.5 more suitable for applications where samples are exposed to light for extended periods, including:

• 流式细胞术和光谱流式细胞术
• 免疫荧光法
• 空间生物学
• Western blotting
• FACS

与绿色和蓝色染料联用实现清晰成像 Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy ®5.5 Conjugate) #90088 (right) compared to concentration-matched Rat Isotype Control (PerCP-Cy®5.5 Conjugate) #79201 (left), co-stained with CD44 (IM7) Rat mAb (violetFluor™ 450 Conjugate) #53289.

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use PerCP-Cy^®5.5 antibody conjugates from CST. 严格且可重复的生产工艺，造就高质量的抗体偶联物——25 年来始终如一的品质标准，值得您的信赖。For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. 每个订单都附有全面的产品文档，并可享专家技术支持。

了解为什么研究人员信任 CST

Explore Our PerCP-Cy^®5.5 Conjugated Antibodies

Survey our catalog of validated PerCP-Cy^®5.5 antibody conjugates, or take advantage of our custom conjugation services and let us build what you need for your research.

CD127/IL-7Rα (A7R34) Rat mAb (PerCP-Cy®5.5 Conjugate) #35094	Ly-6G (1A8) Rat mAb (PerCP-Cy®5.5 Conjugate) #63460	IL2-Rα/CD25 (PC61.5) Rat mAb (PerCP-Cy®5.5 Conjugate) #32325

View PerCP-Cy^®5.5 Conjugated Antibodies

相关或替代荧光团

_{仅供研究使用。不得用于诊断流程。}

_{Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. mFluor is a trademark of AAT Bioquest, Inc. mFluor is manufactured by AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}