Phycoerythrin (PE) Conjugated Antibodies

Get unmatched sensitivity with PE conjugated antibodies, one of the brightest fluorophores available. With a strong yellow-orange emission and excitation by standard laser lines, PE delivers a robust signal ideal for detecting low-abundance markers and rare populations. Its superior brightness and versatility make PE essential for flow cytometry, immunophenotyping, FACS, and more.

Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP® Rabbit mAb (PE Conjugate) #12184 and DRAQ5 #4084 (fluorescent DNA dye).

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属性

The excitation spectrum (dashed yellow line) and emission spectrum (solid yellow line) for PE are shown above. PE is one of the most sensitive fluorophores available, with a near-perfect quantum efficiency. Excitable by standard 488, 532, and 561 nm lasers, PE conjugated antibodies are ideal for flow cytometry and multicolor panels, enabling precise detection of your target.

研究应用

PE is an essential fluorophore for applications requiring high sensitivity, strong fluorescence intensity, and clear signal separation. It is particularly effective for:

• Detailed immunophenotyping of immune cell subsets, ensuring clear and accurate detection of specific populations in complex samples.
• Minimizing spectral overlap and enhancing clarity in multiplexed flow cytometry experiments when paired with other fluorophores like FITC, APC, and PerCP.
• Detection of rare markers and cell populations, making it indispensable for research applications requiring high sensitivity.

PE conjugated antibodies are broadly compatible with varied detection systems making them suitable for multiplexed applications such as:

• 流式细胞术和光谱流式细胞术

• Immunophenotyping

• Western blotting

• FACS

Achieve high-resolution cell sorting with multiplexed workflows Flow cytometric analysis of mouse splenocytes, untreated (left column) or treated with cross-linked anti-CD3 plus anti-CD28 (right column), using Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb (PE Conjugate) #76143 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (bottom row), and co-stained with CD3 (17A2) Rat mAb (FITC Conjugate) #86603.

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use PE conjugated antibodies from CST. 严格且可重复的生产工艺，造就高质量的抗体偶联物——25 年来始终如一的品质标准，值得您的信赖。对于定制或定制化偶联物，我们凭借数十年的经验、全面的生物物理表征和亲本抗体验证来提供具有高特异性和敏感性的产品。每个订单都附有全面的产品文档，并可享专家技术支持。

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Explore Our PE Conjugated Antibodies

Need ready-made PE antibody conjugates? Explore our extensive catalog of validated products, or take advantage of our custom conjugation services and let us build the perfect tool for you.

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate) #5315	Phospho-SLP-76 (Ser376) (D7S1K) Rabbit mAb (PE Conjugate) #16318	PU.1 (E8I8L) Mouse mAb (PE Conjugate) #20251

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