查看特别推荐 >>

HRP Conjugated Antibodies

Accurately detect and visualize a variety of biological targets with CST® Horseradish Peroxidase (HRP) antibody conjugates. Through the catalytic oxidation of various substrates, HRP conjugates produce a colorimetric, chemiluminescent, or fluorescent signal to identify your target while permitting signal amplification. Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate).
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate) #12413.

View HRP Conjugated Antibodies

属性

High Sensitivity: HRP's robust signal amplification allows for highly sensitive detection of low-abundance targets, making it ideal for assays like Western blotting and ELISA.

Ease of use: HRP conjugates deliver rapid and strong signal development, offering simplicity and efficiency for routine lab use and high-throughput applications.

Stability: HRP conjugates are stable, dependable, and easy to store, providing cost-effective, consistent performance across multiple experiments.

Detection speed: HRP's fast enzymatic reaction enables rapid signal development, ideal for time-sensitive assays and applications.

研究应用

The unique enzymatic mode of action for HRP-conjugated antibodies makes them a powerful tool for research:

  • HRP amplifies your output signals, enabling the detection of even low-abundance target molecules.
  • HRP conjugates support colorimetric, chemiluminescent, and fluorescent detection, offering versatility for qualitative (e.g., Western blot) and quantitative (e.g., ELISA) assays.
  • HRP antibody conjugates are compatible with tyramide signal amplification (TSA) to allow the detection of low-abundant targets.
  • HRP-conjugated antibodies are commonly used in assays to detect biomarkers, hormones, and pathogens as well as measure protein concentrations in various biological fluids such as cell culture supernatants and serum.

HRP is broadly compatible with varied detection systems depending on the conjugation type, making it suitable for numerous applications such as:

使用浓度为 0.005 μg/ml 的人 IgG、IgM 和 IgA 的特异度数据。结果表明在 1 μg/ml 浓度下使用的 Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody 和人 IgG 或 IgM 之间没有反应性。
Achieve specific and robust signal amplification


使用浓度为 0.005 μg/ml 的人 IgG、IgM 和 IgA 的特异度数据。Results show no reactivity between Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody #80403 and human IgG or IgM when used at a concentration of 1 μg/ml.

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use HRP conjugated antibodies. A stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. 每个订单都附有全面的产品文档,并可享专家技术支持。

了解为什么研究人员信任 CST

Explore Our HRP Conjugates

Looking for an HRP conjugate to accelerate your research? Explore our extensive catalog of validated products, or let us help you design the perfect solution with our custom conjugation services.

 
使用 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) 对未经和已经钒酸钠(1 mM,30 分钟)处理的 Jurkat 和 3T3/NIH 细胞的提取物进行蛋白质印迹分析。 Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using Sox2 (D6D9) XP® Rabbit mAb #3579 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) (right). 5125_fig01_W_264 Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (HRP Conjugate).

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114

β-Actin (13E5) Rabbit mAb (HRP Conjugate) #5125

View HRP Conjugated Antibodies

相关或替代荧光团

Alkaline phosphatase (AP)

Soybean peroxidase (SBP)