Fluorescein isothiocyanate (FITC) Conjugated Antibodies

Achieve reliable target detection with FITC conjugated antibodies, a widely used conjugation for labeling biomolecules. With bright green emission and excitation achieved with the standard 488 nm laser, FITC is a versatile choice for flow cytometry, immunofluorescence, and microscopy, providing strong fluorescence signals for clear, precise detection.

Confocal immunofluorescent analysis of human CD8+ T cells (left, positive) and MJ [G11] cells (right, negative) using CD8α (RPA-T8) Mouse mAb (FITC Conjugate) #55397 (green). Samples were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue).

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属性

激发：490	发射波长：525
激光线：488 nm	常用滤光片组：530/30
亮度：3	量子产率：0.50

The excitation spectrum (dashed green line) and emission spectrum (solid green line) for FITC are shown above. With bright green emission, FITC provides a strong signal ideal for flow cytometry, immunofluorescence, and fluorescence microscopy, though it is sensitive to pH changes and photobleaching. Its broad compatibility and affordability make it a cornerstone fluorophore for many applications.

研究应用

FITC is a versatile fluorophore ideal for applications requiring clear green fluorescence, broad compatibility, and cost-effective labeling solutions.

FITC is one of the most widely used dyes for antibody and protein conjugation in fluorescence applications. As a result, most instruments are equipped with a 488 nm laser and a corresponding FITC filter setup.
FITC conjugated antibodies provide strong, distinct green signals in immunofluorescence applications, making it easy to visualize proteins or cellular structures in fixed or live-cell imaging.
FITC is an excellent, cost-effective choice for labs needing robust and reproducible results in high-throughput assays.

FITC conjugated antibodies are broadly compatible with varied detection systems, making them suitable for multiplexed applications such as:

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Immunophenotyping
Fluorescence microscopy
FISH
Fluorescent microplate reader assays

Multiplexed tissue imaging made easy

Confocal immunofluorescent analysis of mouse spleen using CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (green), Iba1/AIF-1 (E4O4W) XP^® Rabbit mAb (Alexa Fluor^® 555 Conjugate) #36618 (cyan pseudocolor), and CD45 (D3F8Q)\ Rabbit mAb (Alexa Fluor^® 647 Conjugate) #81143 (red). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue).

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use FITC conjugates from CST. 严格且可重复的生产工艺，造就高质量的抗体偶联物——25 年来始终如一的品质标准，值得您的信赖。对于定制或定制化偶联物，我们凭借数十年的经验、全面的生物物理表征和亲本抗体验证来提供具有高特异性和敏感性的产品。每个订单都附有全面的产品文档，并可享专家技术支持。

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Explore Our FITC Conjugated Antibodies


Ly-6G (1A8) Rat mAb (FITC Conjugate) #88876	CD4 (RM4-5) Rat mAb (FITC Conjugate) #96127	Annexin V-FITC Early Apoptosis Detection Kit #6592