APC-Cy^®7 Conjugated Antibodies

Choose APC-Cy7 antibody conjugates to enhance multicolor flow cytometry and immunophenotyping workflows. The far-red/NIR emission (~785 nm), high brightness, and sensitivity labels low-abundance markers while minimizing background autofluorescence and spectral overlap. Easily excited by 633 or 640 nm lasers, APC-Cy^®7 is ideal for panels requiring precise detection and minimal interference.

Flow cytometric analysis of live mouse peritoneal macrophages using F4/80 (BM8.1) Rat mAb (APC-Cy7 Conjugate) #19233 (right), compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (APC-Cy^®7 Conjugate) #63801 (left)

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属性

The excitation spectrum (dashed red line) and emission spectrum (solid red) for APC-Cy®7 are shown above. This tandem fluorophore combines APC’s brightness with the Cy7 far-red emission to minimize spectral overlap and reduce background autofluorescence. Its large Stokes shift and compatibility with 633 and 640 nm laser lines make APC-Cy^®7 an ideal addition for multicolor flow cytometry panels.

研究应用

APC-Cy^®7 is invaluable in applications that require high sensitivity, clear signal separation, and minimal background interference. It is particularly effective for:

• Targeting highly expressed markers like CD4, CD8, and CD19 for detailed immunophenotyping of immune cell subsets in complex samples such as blood and tissue lysates.
• Multicolor flow cytometry panels, when paired with many of the commonly used blue, green, and orange fluorochromes like Pacific Blue™, FITC, and PE to reduce spectral overlap and compensation needs.
• Sorting specific cell populations based on surface marker expression with minimal crosstalk.

APC-Cy^®7 is widely compatible with diverse detection systems, red laser lines, and filter sets, making it ideal for varied applications such as:

• 流式细胞术和光谱流式细胞术

• Immunophenotyping

• Western blotting

• FACS

Enhance the detection of low abundance targets Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (APC-Cy®7 Conjugate) #18118 (right) compared to concentration-matched Rat Isotype Control (APC-Cy®7 Conjugate) (left), co-stained with CD44 (IM7) Rat mAb (FITC Conjugate) #75122.

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use APC-Cy^®7 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. 对于定制或定制化偶联物，我们凭借数十年的经验、全面的生物物理表征和亲本抗体验证来提供具有高特异性和敏感性的产品。每个订单都附有全面的产品文档，并可享专家技术支持。

了解为什么研究人员信任 CST

Explore Our APC-Cy^®7 Conjugated Antibodies

CD45R/B220 (RA3-6B2) Rat mAb (APC-Cy7® Conjugate) #31953	F4/80 (BM8.1) Rat mAb (APC-Cy7® Conjugate) #19233	Ly-6G (1A8) Rat mAb (APC-Cy7® Conjugate) #79038

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