Alexa Fluor® 350 Conjugated Antibodies

Differentiate your molecular targets from other fluorophores using the intense blue emission of Alexa Fluor 350 antibody conjugates. Alexa Fluor 350 can be excited in the ultraviolet spectrum, is photostable, avoids most blue-green autofluorescence, and is ideal for high-abundance targets.

Flow cytometric analysis of fixed/permeabilized MCF7 cells (blue, negative) and SNB-19 cells (green, positive) using Vimentin (D21H3) XP ^® Rabbit mAb (Alexa Fluor^® 350 Conjugate) #62006 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP ^® Isotype Control (Alexa Fluor^® 350 Conjugate) #34051 (dashed lines).

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属性

The excitation spectrum (dashed blue line) and emission spectrum (solid blue) of Alexa Fluor 350 are shown above. Selecting fluorophores with minimal excitation and emission spectral overlap yields the best results when multiplexing. Alexa Fluor 350 has a broad excitation range, allowing it to be excited by ultraviolet (330 nm) and conventional violet lasers (355 nm).

研究应用

There are many characteristics of Alexa Fluor 350 that can be leveraged for maximum impact in your research:

• The deep blue of Alexa Fluor 350 is ideal for tagging background or abundant proteins or cells for multiplexed flow cytometry with the bright green of Alexa Fluor^® 488.
• Available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 350 has broad compatibility with several detection systems and is suitable for numerous multiplexed applications, including:

• 流式细胞术
• 免疫荧光法
• Western blotting
• FRET
• Super-resolution microscopy

High performance in flow cytometry Flow cytometric analysis of fixed/permeabilized 293T cells, mock transfected (blue) or transfected with a construct expressing an HA-tagged protein (green), using HA-Tag (C29F4) Rabbit mAb (Alexa Fluor® 350 Conjugate) #34888 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor® 350 Conjugate) #34051 (dashed lines).

品质承诺

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 350 conjugates from CST. 严格且可重复的生产工艺，造就高质量的抗体偶联物——25 年来始终如一的品质标准，值得您的信赖。对于定制或定制化偶联物，我们凭借数十年的经验、全面的生物物理表征和亲本抗体验证来提供具有高特异性和敏感性的产品。每个订单都附有全面的产品文档，并可享专家技术支持。

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探索我们的 Alexa Fluor 350 偶联抗体

Looking for Alexa Fluor 350-conjugated primary antibodies? Browse our range of validated products, or use our custom conjugation services to create the perfect solution tailored to your needs.

Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 350 Conjugate) #34051	Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Alexa Fluor® 350 Conjugate) #69217	Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 350 Conjugate) #48319

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相关或替代荧光团

_{仅供研究使用。不得用于诊断流程。}

_{Cell Signaling Technology, CST and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}