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SignalSilence® Prolactin Receptor siRNA I

SignalSilence^® Prolactin Receptor siRNA I #13822

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Supporting Data

REACTIVITY

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

CST recommends transfection with 100 nM SignalSilence^® Prolactin Receptor siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Storage

Prolactin Receptor siRNA I is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence^® Prolactin Receptor siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit prolactin receptor expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Background

Prolactin receptor (PRLR) is a single-pass transmembrane receptor that mediates the actions of prolactin, a peptide hormone secreted by the anterior pituitary. PRLR is a type 1 cytokine receptor that is best known for promoting lactation in mammals, but which is also implicated in osmoregulation, metabolism, and immune system function (1). Research studies suggest that PRLR activation may promote tumor growth (2). Prolactin signaling via PRLR can activate multiple signal transduction pathways in breast cancer cells, including the Jak/Stat, PI3K/Akt, and MAPK pathways, leading to both pro-proliferative and anti-apoptotic downstream effects (3,4). Nine isoforms of PRLR have been identified, with the canonical (long) isoform primarily responsible for the pro-oncogenic effects of PRLR in some cancer cell lines (3). Much less is known about the functions of the other prolactin receptor isoforms. Defining the precise role of PRLR in promoting growth of breast cancer and other tumor types remains an area of active investigation (2).

Database Links

UniProt ID: P16471

Entrez-Gene Id: 5618

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

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SignalSilence® Prolactin Receptor siRNA I #13822