WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) #89348
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 51-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) recognizes endogenous levels of total WT1 protein.
This antibody is recommended for the detection of fusion proteins containing the C-terminus region of WT1 found in desmoplastic small round cell tumors by immunohistochemistry. For the detection of wild-type WT1, it is recommended to use WT1 (D8I7F) XP® Rabbit mAb #83535.
Non-specific staining was observed in kidney, liver, and small intestine by immunohistochemistry.
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post-translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post-translational modifications of the protein (9).
- Royer-Pokora, B. et al. (2004) Am J Med Genet A 127A, 249-57.
- Kohsaka, T. et al. (1999) Hum Mutat 14, 466-70.
- Little, M. et al. (2000) Hum Mutat 15, 389.
- Takata, A. et al. (2000) J Med Genet 37, 698-701.
- Suri, M. et al. (2007) Am J Med Genet A 143A, 2312-20.
- Toska, E. and Roberts, S.G. (2014) Biochem J 461, 15-32.
- Yang, L. et al. (2007) Leukemia 21, 868-76.
- Weiss, T.C. and Romaniuk, P.J. (2009) Biochemistry 48, 148-55.
- Haber, D.A. et al. (1991) Proc Natl Acad Sci USA 88, 9618-22.
限制使用
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