Wnt/β-Catenin Activated Targets Antibody Sampler Kit #8655
Product Information
Kit Usage Information
Protocols
- 2203: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic
- 2230: Western Blotting, Immunofluorescence, Flow
- 2978: Western Blotting, Immunohistochemistry (Paraffin)
- 3570: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, Flow*
- 3801: Western Blotting, Immunohistochemistry (Paraffin)
- 5605: Western Blotting, Immunofluorescence
- 7074: Western Blotting
- 7076: Western Blotting
- 8198: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 9165: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow Triton Permeabilization (Rabbit), ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
Product Description
The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform two western blots per primary.
Background
The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11).
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- Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
- Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
- Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
- Zeilstra, J. et al. (2008) Cancer Res 68, 3655-61.
- Shtutman, M. et al. (1999) Proc Natl Acad Sci U S A 96, 5522-7.
- Mann, B. et al. (1999) Proc Natl Acad Sci U S A 96, 1603-8.
- Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6.
- Boon, E.M. et al. (2002) Cancer Res 62, 5126-8.
- Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8.
- Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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