Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Visinin-Like Protein 1 (D9L6L) Rabbit mAb #49468
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Visinin-like Protein 1 (D9L6L) Rabbit mAb recognizes endogenous levels of total Visinin-like Protein 1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg150 of human Visinin-like Protein 1 protein.
Background
Visinin-like Protein 1, also called VILIP-1 and VSNL1, is a calcium-sensing protein in the central nervous system. Visinin-like Protein 1 exhibits a widespread distribution with high expression in the CNS, and lower expression levels in some peripheral tissues (1). Visinin-like Protein 1 responds to increased intracellular calcium concentration by translocating from the cytoplasm to membranes through calcium-dependent myristoylation at its N-terminus that allows interaction with membranes (2). This change in localization has been proposed to facilitate the activation of compartment-specific signal transduction for the selective activation of downstream signaling cascades (3,4). In Alzheimer’s disease, Visinin-like Protein 1 expression is decreased in the brain, including a reduced number of Visinin-like Protein 1 immunoreactive neurons (5); however, the presence of the protein is increased in the CSF (6), suggesting that it is released from neurons during insults.
- Gierke, P. et al. (2004) Biochem Biophys Res Commun 323, 38-43.
- Kobayashi, M. et al. (1993) J Biol Chem 268, 18898-904.
- Spilker, C. et al. (2002) J Neurosci 22, 7331-9.
- Braunewell, K.H. et al. (1997) J Neurochem 68, 2129-39.
- Bernstein, H.G. et al. (1999) J Neurocytol 28, 655-62.
- Lee, J.M. et al. (2008) Clin Chem 54, 1617-23.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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