R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
USP22 (F4M4V) Rabbit mAb #52293
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
USP22 (F4M4V) Rabbit mAb recognizes endogenous levels of total USP22 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu457 of human USP22 protein.
Background
Ubiquitin-specific-processing protease 22 (USP22) is a deubiquitinating enzyme that, along with TXN7, ATXN7L3, and ENY2, forms the deubiquitination module of the human SAGA complex (1,2). SAGA is capable of deubiquitinating H2A and H2B in opposition to polycomb activity (3,4). In addition to histone targets, USP22 deubiquitinates and stabilizes SirT1, which in turn deacetylates and suppresses p53 to repress apoptosis (5). Regulation of SirT1 by USP22 has also been shown to affect STAT signaling (6). USP22 plays a role in double-stranded break repair and antibody class switching (7,8). USP22 has also been shown to occupy the SOX2 promoter, blocking transcription and thus regulating pluripotency (9). Many cancers have elevated levels of USP22, which can promote cancer progression and metastasis (10-12).
- Zhao, Y. et al. (2008) Mol Cell 29, 92-101.
- Zhang, X.Y. et al. (2008) Mol Cell 29, 102-11.
- Zhang, X.Y. et al. (2008) Cell Cycle 7, 1522-4.
- Lang, G. et al. (2011) Mol Cell Biol 31, 3734-44.
- Lin, Z. et al. (2012) Mol Cell 46, 484-94.
- Ao, N. et al. (2014) Cell Physiol Biochem 33, 1863-75.
- Ramachandran, S. et al. (2016) Cell Rep 15, 1554-1565.
- Li, C. et al. (2018) Nat Commun 9, 1006.
- Sussman, R.T. et al. (2013) J Biol Chem 288, 24234-46.
- Liu, Y.L. et al. (2010) J Gastroenterol Hepatol 25, 1800-5.
- Liu, Y.L. et al. (2011) J Surg Oncol 103, 283-9.
- Zhang, Y. et al. (2011) J Cancer Res Clin Oncol 137, 1245-53.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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