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  3. USP18 (D4E7) Rabbit Monoclonal Antibody
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USP18 (D4E7) Rabbit Monoclonal Antibody #4813

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 34, 39
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    USP18 (D4E7) Rabbit Monoclonal Antibody detects endogenous levels of total USP18 protein. The doublet band detected by western blot represents full length (39 kDa) and amino-terminal deleted derivative of USP18 (8).

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro45 of human USP18 protein.

    Background

    Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes (1,2). USP18 (also known as UBP43) is a deubiquitinase best known for catalyzing the removal of ISG15, an interferon-regulated ubiquitin-like protein, from conjugated proteins (3). Removal of ISG15 from target proteins by the USP18 peptidase maintains the critical cellular balance of ISG15-conjugated proteins important for normal development and brain function (4,5). Following induction by IFN or LPS (6), USP18 binds the INF receptor subunit IFNAR2 and inhibits signal transduction through the Jak/Stat pathway (7). USP18 regulation of IFN signaling inhibits IFN-mediated apoptosis and does not necessarily rely on USP18 peptidase activity (8). As the therapeutic use of recombinant IFN can lead to refractory IFN signaling and a less effective response, the combination of IFN treatment and regulation of USP18 expression may produce a more positive outcome (9).

    Alternate Names

    43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; ISG43; PTORCH2; ubiquitin specific peptidase 18; ubiquitin specific protease 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; ubl thiolesterase 18; UBP18; UBP43; USP18

    Database Links

    UniProt ID: Q9UMW8


    Entrez-Gene Id: 11274


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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