Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UFL1 (F5P6L) Rabbit mAb (BSA and Azide Free) #18040
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This product is the carrier free version of product #96396. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #96396
For standard formulation of this product see product #96396
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
UFL1 (F5P6L) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total UFL1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFL1 protein.
Background
The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4).
- Tatsumi, K. et al. (2010) J Biol Chem 285, 5417-27.
- Gerakis, Y. et al. (2019) Trends Cell Biol 29, 974-986.
- Jiang, Q. et al. (2023) Front Endocrinol (Lausanne) 14, 1123124.
- Jing, Y. et al. (2022) Cancers (Basel) 14, 3501.
- Liu, J. et al. (2017) Nat Commun 8, 14186.
- Walczak, C.P. et al. (2019) Proc Natl Acad Sci USA 116, 1299-1308.
- Liu, J. et al. (2020) Nat Cell Biol 22, 1056-1063.
- Lee, L. et al. (2021) Sci Adv 7, eabc7371.
- Qin, B. et al. (2019) Nat Commun 10, 1242.
- Yoo, H.M. et al. (2014) Mol Cell 56, 261-274.
- Yang, R. et al. (2019) Development 146, dev169235. doi: 10.1242/dev.169235.
- Ishimura, R. et al. (2022) Nat Commun 13, 7857.
- Liang, J.R. et al. (2020) Cell 180, 1160-1177.e20.
- DeJesus, R. et al. (2016) Elife 5, e17290. doi: 10.7554/eLife.17290.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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