R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Ubc12 (D13D7) Rabbit mAb #5641
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Ubc12 (D13D7) Rabbit mAb detects endogeneous levels of total Ubc12 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human Ubc12 protein.
Background
Similar to ubiquitin, NEDD8 is covalently linked to target proteins through an enzymatic cascade composed of NEDD8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for NEDD8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 are cullin-based RING E3 ligases. Neddylation of cullin isoforms activates the related ubiquitin E3 complex by promoting its interaction with a cognate ubiquitin-E2 ligase (6-7). Neddylation of Cul-1 complexes containing βTrCP and SKP2 has been shown to be required for controlling the stability of important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11).
- Huang, D.T. et al. (2007) Nature 445, 394-8.
- Huang, D.T. et al. (2005) Mol Cell 17, 341-50.
- Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14.
- Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42.
- Wada, H. et al. (2000) J Biol Chem 275, 17008-15.
- Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8.
- Kawakami, T. et al. (2001) EMBO J 20, 4003-12.
- Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84.
- Wu, K. et al. (2002) J Biol Chem 277, 516-27.
- Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9.
- Herrmann, J. et al. (2007) Circ Res 100, 1276-91.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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