R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Tyro3 (D38C6) Rabbit mAb (BSA and Azide Free) #64590
Filter:
- WB
- ELISA
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110, 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This product is the carrier free version of product #5585. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #5585
For standard formulation of this product see product #5585
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Tyro3 (D38C6) Rabbit mAb (BSA and Azide Free) detects endogeneous levels of total Tyro3 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr874 of human Tyro3 protein.
Background
Tyro3 is a receptor tyrosine kinase belonging to the TAM subfamily (Tyro3, Axl, and Mer). All three members have similar domain structures composed of an extracellular region with 2 Ig-like domains, followed by 2 FNII-like domains, a single transmembrane region, and a cytoplasmic tyrosine kinase domain (1). The natural ligand for Tyro3, as well as Axl and Mer, is GAS6 (growth arrest specific gene 6) (1,2). Expression pattern and target knockout data indicate an important role of Tyro3 in apoptotic cell phagocytosis of dendritic cells and macrophages (3), NK cell differentiation (4), reproductive neuron survival and migration (5), osteoclast stimulation (6,7), as well as cortical and hippocampal neuron function (8). Both MAPK and PI3K pathways have been suggested as downstream targets of Tyro3 activation (7,8). Tyro3 has also been shown to be correlated to melanoma tumorigenesis, likely through its regulatory role in the expression of oncogenic microphthalmia-associated transcription factor (MITF) (9).
- Binder, M.D. and Kilpatrick, T.J. (2009) Neurosignals 17, 277-87.
- Nagata, K. et al. (1996) J Biol Chem 271, 30022-7.
- Seitz, H.M. et al. (2007) J Immunol 178, 5635-42.
- Caraux, A. et al. (2006) Nat Immunol 7, 747-54.
- Pierce, A. et al. (2008) Mol Endocrinol 22, 2481-95.
- Nakamura, Y.S. et al. (1998) Stem Cells 16, 229-38.
- Katagiri, M. et al. (2001) J Biol Chem 276, 7376-82.
- Prieto, A.L. et al. (2007) Neuroscience 150, 319-34.
- Zhu, S. et al. (2009) Proc Natl Acad Sci U S A 106, 17025-30.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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