TRXR2/TXNRD2 (3F2-E12-F10) Mouse mAb #12029
Filter:
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 56 |
| Source/Isotype | Mouse IgG3 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRXR2/TXNRD2 (3F2-E12-F10) Mouse mAb recognizes endogenous levels of total TRXR2/TXNRD2 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human TRXR2/TXNRD2 protein.
Background
Thioredoxin reductases are selenoproteins that are critical for maintaining cellular redox balance and eliminating reactive oxygen species (ROS) (1-4). There are three known mammalian thioredoxin reductases: TRXR1/TXNRD1 (cytosolic), TRXR2/TXNRD2 (mitochondrial), and TRXR3/TXNRD3 (testis-specific) (1). TRXR2 may function to protect against tumor necrosis factor-α (TNF-α)-mediated ROS generation (5). TRXR2 is critical for normal heart development and function (1,4). TRXR2 KO mice experience embryonic lethality, while mitochondrial dysfunction seen in heart-specific TRXR2 KO mice results in congestive heart failure (4). In humans, certain rare mutations in TRXR2 have been linked to dilated cardiomyopathy (1). A recent study in the EPIC-Heidelberg cohort shows that serum selenium levels, in concert with certain SNPs in TRXR2 and other selenoproteins, can influence prostate cancer risk (6).
- Sibbing, D. et al. (2011) Eur Heart J 32, 1121-33.
- Gasdaska, P.Y. et al. (1999) FEBS Lett 442, 105-11.
- Miranda-Vizuete, A. et al. (1999) Eur J Biochem 261, 405-12.
- Conrad, M. et al. (2004) Mol Cell Biol 24, 9414-23.
- Hansen, J.M. et al. (2006) Toxicol Sci 91, 643-50.
- Méplan, C. et al. (2012) PLoS One 7, e48709.
限制使用
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