R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TopBP1 (D8G4L) Rabbit mAb #14342
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 170 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Protocol
Specificity / Sensitivity
TopBP1 (D8G4L) Rabbit mAb recognizes endogenous levels of total TopBP1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu27 of human TopBP1 protein.
Background
Topoisomerase II Binding Protein 1 (TopBP1) contains eight BRCT domains, which facilitate interaction with various proteins, phosphopeptides, and DNA. Through these interactions, TopBP1 functions in the regulation of DNA replication, DNA repair, checkpoint control, and transcription (1). TopBP1 contacts the checkpoint kinase ATR and its binding partner ATRIP, and induces ATR and Chk1 activation in collaboration with claspin (2,3). Activation of ATR is dependent on recruitment of TopBP1 through the MRN (MRE11-RAD50-NBS1) complex, and the 911 (RAD9-RAD1-HUS1) complex is required for full activation (4). TopBP1 stabilizes Bloom syndrome helicase (BLM) during S phase of the cell cycle to suppress sister chromatin exchange and maintain genome stability (5).
TopBP1 also regulates initiation of DNA replication along with the DNA replication factor treslin (6,7). TopBP1 has been shown to prevent replication associated DNA damage during neurogenesis (8), and to interact with mutant p53, mediating mutant p53 gain-of-function activity such as growth promotion and resistance to chemotherapeutic drugs (9). Phosphorylation of TopBP1 at Ser1159 by Akt regulates TopBP1 oligomerization and function in E2F1-dependent transcriptional regulation (10).
TopBP1 also regulates initiation of DNA replication along with the DNA replication factor treslin (6,7). TopBP1 has been shown to prevent replication associated DNA damage during neurogenesis (8), and to interact with mutant p53, mediating mutant p53 gain-of-function activity such as growth promotion and resistance to chemotherapeutic drugs (9). Phosphorylation of TopBP1 at Ser1159 by Akt regulates TopBP1 oligomerization and function in E2F1-dependent transcriptional regulation (10).
- Garcia, V. et al. (2005) DNA Repair (Amst) 4, 1227-39.
- Lindsey-Boltz, L.A. and Sancar, A. (2011) J Biol Chem 286, 19229-36.
- Burrows, A.E. and Elledge, S.J. (2008) Genes Dev 22, 1416-21.
- Duursma, A.M. et al. (2013) Mol Cell 50, 116-22.
- Wang, J. et al. (2013) Mol Cell 52, 667-78.
- Kumagai, A. et al. (2010) Cell 140, 349-59.
- Boos, D. et al. (2013) Science 340, 981-4.
- Lee, Y. et al. (2012) Nat Neurosci 15, 819-26.
- Liu, K. et al. (2011) Mol Cell Biol 31, 4464-81.
- Liu, K. et al. (2013) Mol Cell Biol 33, 4685-700.
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