Tat (8C2) Mouse mAb #2547
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Inquiry Info. # 2547
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Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 14 |
| Source/Isotype | Mouse IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Tat (8C2) Mouse mAb detects recombinant Tat protein and Tat-tagged overexpressed protein. It does not cross-react with any other proteins.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic HIV-1 Tat-tag peptide.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
A small peptide derived from the HIV-1 Tat protein has been used to internalize various types of molecules, from small particles to proteins, peptides and nucleic acids, into the cells. The 'transduction domain' or region conveying the cell penetrating properties of HIV Tat-derived peptide appears to be confined to a small (9 amino acid) stretch of basic amino acids, with the sequence RKKRRQRRR. The way this peptide enters cells is still under investigation (1).
A small peptide derived from the HIV-1 Tat protein has been used to internalize various types of molecules, from small particles to proteins, peptides and nucleic acids, into the cells. The 'transduction domain' or region conveying the cell penetrating properties of HIV Tat-derived peptide appears to be confined to a small (9 amino acid) stretch of basic amino acids, with the sequence RKKRRQRRR. The way this peptide enters cells is still under investigation (1).
Alternate Names
tag
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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