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74798
Synaptic Neuron Marker Antibody Sampler Kit
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Synaptic Neuron Marker Antibody Sampler Kit #74798

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Western blot analysis of extracts from mouse and rat brain using Synaptotagmin-1 (D33B7) Rabbit mAb.
Western blot analysis of extracts from Neuro-2a, NIH/3T3, and mouse brain, using Complexin-1/2 (D8A6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from mouse and rat brain using PSD95 (D74D3) XP® Rabbit mAb.
Western blot analysis of extracts from mouse and rat tissue using Synaptophysin (D8F6H) XP® Rabbit mAb (upper) and B-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb.
Western blot analysis of extracts from mouse and rat brain using SynGAP (D78B11) Rabbit mAb.
Western blot analysis of extracts from mouse neonatal brain, C2C12 cells and rat fetal brain using SHANK3 (D5K6R) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from mouse and rat brain tissues using Bassoon (D63B6) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Confocal immunofluorescent analysis of mouse retina using Synaptotagmin-1 (D33B7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1/2 (D8A6E) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunohistochemical analysis of paraffin-embedded rat retina using PSD95 (D74D3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded Neuro-2a cell pellet (left, positive) or C2C12 cell pellet (right, negative) using Synaptophysin (D8F6H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human cerebellum using Synapsin-1 (D12G5) XP® Rabbit mAb.
Western blot analysis of striatal synaptosome extracts prepared from postnatal day 14 wild-type and SHANK3 -/- mice using SHANK3 (D5K6R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Wild-type and SHANK3 -/- synaptosomal lysates were kindly provided by Tobias Kaiser and Dr. Guoping Feng (MIT, Cambridge, MA).
Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus, and retina using Bassoon (D63B6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunoprecipitation of complexin-1/2 from Neuro-2A cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Complexin-1/2 (D8A6E) Rabbit mAb. Western blot analysis was performed using Complexin-1/2 (D8A6E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Synaptophysin (D8F6H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse cortex using Synapsin-1 (D12G5) XP® Rabbit mAb.
Confocal immunofluorescent analysis of rat cerebellum (left), hippocampus (middle), and striatum (right) using SHANK3 (D5K6R) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of Neuro-2a (left) and NIH/3T3 (right) cells, using Complexin-1/2 (D8A6E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse colon using Synaptophysin (D8F6H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat retina using Synapsin-1 (D12G5) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Synaptophysin (D8F6H) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Synaptophysin (D8F6H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Synaptophysin (D8F6H) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse retina using Synaptophysin (D8F6H) XP® Rabbit mAb (green), Neurofilament-L (DA2) Mouse mAb #2835 (yellow) and Lamin A/C (4C11) Mouse mAb #4777 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse brain using Synaptophysin (D8F6H) XP® Rabbit mAb (green), Neurofilament-L (DA2) Mouse mAb #2835 (yellow) and Lamin A/C (4C11) Mouse #4777 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
To Purchase # 74798T
Cat. # Size Price Inventory
74798T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PSD95 (D74D3) XP® Rabbit mAb 3409 20 µl
  • WB
  • IHC
H M R 95 Rabbit IgG
Synaptophysin (D8F6H) XP® Rabbit mAb 36406 20 µl
  • WB
  • IHC
  • IF
H M R 38 Rabbit IgG
Bassoon (D63B6) Rabbit mAb 6897 20 µl
  • WB
  • IF
M R 420 Rabbit IgG
Complexin-1/2 (D8A6E) Rabbit mAb 28070 20 µl
  • WB
  • IP
  • IF
H M R 14-16 Rabbit IgG
Synaptotagmin-1 (D33B7) Rabbit mAb 14558 20 µl
  • WB
  • IP
  • IF
H M R 60 Rabbit IgG
SHANK3 (D5K6R) Rabbit mAb 64555 20 µl
  • WB
  • IP
  • IF
H M R 220 Rabbit IgG
Synapsin-1 (D12G5) XP® Rabbit mAb 5297 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 77 Rabbit IgG
SynGAP (D78B11) Rabbit mAb 5540 20 µl
  • WB
  • IP
M R 140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Synaptic Neuron Marker Antibody Sampler Kit provides an economical means of detecting presynaptic and postsynaptic proteins by western blot. This kit includes enough primary antibodies to perform at least two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Synaptic Neuron Marker Antibody Sampler Kit detects endogenous levels of its target protein. Synapsin-1 (D12G5) XP® Rabbit mAb detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b. Bassoon (D63B6) Rabbit mAb recognizes endogenous levels of total bassoon protein. Some background staining was observed in muscle tissue and the choroid plexus by confocal immunofluorescent analysis. Synaptotagmin-1 (D33B7) Rabbit mAb recognizes endogenous levels of total synaptotagmin-1 protein. This antibody may also cross-react with an unidentified protein of approximately 45 kDa.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly299 of human Synaptophysin protein, Gln1217 of human bassoon protein, Pro125 of human complexin-2 protein, Arg400 of human synaptotagmin-1 protein, Ile1210 of human SHANK3 protein, Gln483 of human synapsin-1 protein, Arg732 of human SynGAP protein, and the amino-terminal of human PSD95 protein.

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synapsin-1 is a neuronal phospho-protein localized to presynaptic terminals. Synapsin-1 plays an important role in synapse formation, neurotransmitter regulation, and regulation of synaptic vesicle fusion and trafficking (2,3). Synaptotagmin-1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (4). Complexin isoforms 1 and 2 are small synaptic proteins that bind to SNARE complexes, responsible for regulating exocytosis and synaptic vesicle fusion (5). SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP interacts with the PDZ domains of PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (6). PSD95 is involved in the assembly and function of the postsynaptic density (PSD) complex (7,8). SHANK proteins act as scaffolds at the neuronal PSD (9), where they play a critical role in PSD assembly of excitatory synapses during development (10). Bassoon (BSN) is a scaffolding protein component of the synaptic ribbon and of the cytomatrix at the active zones of both excitatory and inhibitory synapses with a presumptive role in orchestrating events of the synaptic vesicle cycle (11-13). Together, these proteins can be used to measure presynaptic and postsynaptic proteins and synaptic development under normal and disease conditions.

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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