Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SP100 (F1H6X) Rabbit mAb #80075
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80, 90, 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SP100 (F1H6X) Rabbit mAb recognizes endogenous levels of total SP100 protein. This antibody recognizes 7 isoforms of SP100, including SP100-HMG, SP100-A, SP100-B, and SP100-C. This antibody detects a 20 kDa protein of unknown identity in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human SP100 protein.
Background
Speckled protein 100 kDa (SP100), also known as SP100 nuclear antigen, was first identified as an antigen reactive with antibodies from patients with autoimmune disorders (1,2). The nuclear distribution of SP100 predominantly colocalizes with promyelocytic leukemia-nuclear bodies (PML-NBs) (3), which function as structures that regulate several diverse cellular functions, including gene regulation, DNA-damage responses, and apoptosis (4-6). This protein contains several isoforms, and the major isoform SP100-A has a calculated molecular weight of 54 kDa but exerts aberrant electrophoretic mobilities in SDS-polyacrylamide gel electrophoresis of approximately 100 kDa (1,2). SP100 has also been shown to undergo extensive post-translational modifications (PTM), including phosphorylation, ubiquitination, and SUMOylation. Moreover, PML-NBs play an important role in the PTM status of SP100, since they are thought to serve as hotspots for SUMOylation reactions, and PML depletion reduces SP100 SUMOylation (7-9).
- Szostecki, C. et al. (1987) Clin Exp Immunol 68, 108-16.
- Szostecki, C. et al. (1990) J Immunol 145, 4338-47.
- Sternsdorf, T. et al. (1995) Scand J Immunol 42, 257-68.
- Gurrieri, C. et al. (2004) J Natl Cancer Inst 96, 269-79.
- Bernardi, R. and Pandolfi, P.P. (2007) Nat Rev Mol Cell Biol 8, 1006-16.
- Scherer, M. and Stamminger, T. (2016) J Virol 90, 5850-5854.
- Everett, R.D. et al. (2006) J Virol 80, 7995-8005.
- Everett, R.D. et al. (2008) J Virol 82, 2661-72.
- Tavalai, N. et al. (2011) J Virol 85, 9447-58.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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