R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SLC25A37 (F6W1R) Rabbit mAb #44667
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SLC25A37 (F6W1R) Rabbit mAb recognizes endogenous levels of total SLC25A37 protein. This antibody detects bands of unknown origin at 60 and 70 kDa in some cell lines by western blot.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of human SLC25A37 protein.
Background
Mitochondria require iron import for heme and iron-sulfur cluster biogenesis for cellular respiration (reviewed in 1). Perturbation of iron homeostasis can manifest in several pathological conditions. Mitoferrin-1 (SLC25A37) and mitoferrin-2 (SLC25A28) are homologous members of the solute carrier family that are important for iron import into the mitochondria (2). Combinatorial CRISPR screening identified SLC25A37 and the mitochondrial glutathione transporter as important regulators supporting oxidative phosphorylation (3). Mitochondrial depolarization results in a PINK1/Parkin-dependent decrease in SLC25A37 and SLC25A28, and deletion of PINK and Parkin can accelerate K-Ras driven pancreatic tumorigenesis with an increase in mitochondrial iron accumulation (4). Deletion of SLC25A37 homologue in zebrafish inhibited erythroid maturation (5). Decreased expression of SLC25A37 has been described in erythropoietin protoporphyria, in which patients have an accumulation of protoporphyrins in red blood cells (6). Decreased expression of SLC25A37 has also been identified as a risk factor in major depressive disorder (7).
- Dietz, J.V. et al. (2021) Cells 10, 2198. doi: 10.3390/cells10092198.
- Paradkar, P.N. et al. (2009) Mol Cell Biol 29, 1007-16.
- Shi, X. et al. (2022) Nat Commun 13, 2483.
- Li, C. et al. (2018) Dev Cell 46, 441-455.e8.
- Shaw, G.C. et al. (2006) Nature 440, 96-100.
- Wang, Y. et al. (2011) Exp Hematol 39, 784-94.
- Huo, Y.X. et al. (2016) J Psychiatr Res 83, 168-175.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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