R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Semaphorin 3B (D5A2P) Rabbit mAb #13299
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Semaphorin 3B (D5A2P) Rabbit mAb recognizes endogenous level of total semaphorin 3B protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln717 of human semaphorin 3B protein.
Background
Semaphorins are a family of cell surface and secreted proteins initially recognized as axon guidance factors that control the development of the central nervous system (1). They are involved in cell migration, angiogenesis, and immune responses (2-6). Based on protein structure, there are eight classes of semaphorins. Class 3-7 semaphorins are expressed in vertebrates. Semaphorin 3 subfamily members are the only secreted semaphorins in vertebrates. There are seven semaphorin 3 proteins and their receptors include neuropilins and the type A/D family plexins (7-9).
Semaphorin 3B functions as a tumor suppressor, as research studies have shown that it is deleted or inactivated in lung and breast cancer (10,11). Overexpression of semaphorin 3B inhibits tumor cell proliferation and causes apoptosis (12,13). Semaphorin 3B also inhibits angiogenesis (14). Semaphorin 3B loses its activity upon cleavage by furin-like pro-protein convertases (14).
Semaphorin 3B functions as a tumor suppressor, as research studies have shown that it is deleted or inactivated in lung and breast cancer (10,11). Overexpression of semaphorin 3B inhibits tumor cell proliferation and causes apoptosis (12,13). Semaphorin 3B also inhibits angiogenesis (14). Semaphorin 3B loses its activity upon cleavage by furin-like pro-protein convertases (14).
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- Tomizawa, Y. et al. (2001) Proc Natl Acad Sci U S A 98, 13954-9.
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- Castro-Rivera, E. et al. (2004) Proc Natl Acad Sci U S A 101, 11432-7.
- Varshavsky, A. et al. (2008) Cancer Res 68, 6922-31.
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