R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RIF1 (D2F2M) Rabbit mAb #95558
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 274 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RIF1 (D2F2M) Rabbit mAb recognizes endogenous levels of total RIF1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala400 of human RIF1 protein.
Background
The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its effect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double-strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5' end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-8). Oct-4 and Smad3 modulate RIF1 expression in mouse embryonic stem cells, and RIF1 may regulate embryonic stem cell stability during cell proliferation (9). Inhibition of ATR or CHK1 activity induces CDK1-mediated phosphorylation of RIF1 at serine 2205 (human)/serine 2153 (mouse), leading to firing of dormant origins of DNA replication during S phase (10,11).
- Miller, K.M. et al. (2005) EMBO J 24, 3128-35.
- Hayano, M. et al. (2012) Genes Dev 26, 137-50.
- Cornacchia, D. et al. (2012) EMBO J 31, 3678-90.
- Yamazaki, S. et al. (2012) EMBO J 31, 3667-77.
- Zimmermann, M. et al. (2013) Science 339, 700-4.
- Di Virgilio, M. et al. (2013) Science 339, 711-5.
- Chapman, J.R. et al. (2013) Mol Cell 49, 858-71.
- Escribano-Díaz, C. et al. (2013) Mol Cell 49, 872-83.
- Li, P. et al. (2015) Cell Death Dis 6, e1588.
- Moiseeva, T.N. et al. (2019) Proc Natl Acad Sci U S A 116, 13374-13383.
- Sugitani, N. et al. (2022) Cell Rep 40, 111371.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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