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  3. QKI (E7O4A) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

QKI (E7O4A) Rabbit Monoclonal Antibody #23065

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  • WB
  • IP
  • IF
  • eCLIP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 38, 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • eCLIP-eCLIP 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Frozen) 1:400 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
    eCLIP 1:200
    For more information about the RBP-eCLIP service please visit Eclipsebio.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    QKI (E7O4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total QKI protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro11 of human QKI protein.

    Background

    Quaking (QKI) is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins (1,2). Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors (3). QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice (2). QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations (4). The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance (4,5). QKI has also been implicated in schizophrenia and oligodendrocyte differentiation (6). QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling (7).

    Alternate Names

    DKFZp586I0923; HKQ; homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; HqkI; KH domain-containing RNA-binding protein QKI; Protein quaking; QK; QK1; QK3; QKI; QKI, KH domain containing RNA binding; QKI/LOC100132735 fusion; quaking homolog, KH domain RNA binding; quaking homolog, KH domain RNA binding (mouse); RNA binding protein HQK

    Database Links

    UniProt ID: Q96PU8


    Entrez-Gene Id: 9444


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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