Name: PTBP1 (E5O2S) Rabbit Monoclonal Antibody
Price: 351.00 USD
Availability: InStock

Filter:

WB
IP
IHC

Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	57, 59
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

PTBP1 (E5O2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human PTBP1 protein.

Background

PTBP1 and PTBP2 are highly related pre-mRNA binding proteins that silence the splicing of alternative exons (1,2). While PTBP1 is widely expressed, PTBP2, also known as neural PTB or nPTB, is expressed in post-mitotic neurons. In other cell types, PTBP2 transcripts are alternatively spliced by PTBP1, which leads to nonsense-mediated decay (3). PTBP1 and 2 bind to similar regions across the transcriptome, but PTBP1 has a stronger repressive property likely due to cofactors such as Raver1 and Matrin3 (4-6). Overexpression of PTBPs in various cancers has been observed, leading to alternative splicing of key proteins in oncogenic pathways (6-9).

Alternate Names

57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; HNRNP-I; HNRNPI; HNRPI; MGC10830; MGC8461; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); polypyrimidine tract binding protein 1; Polypyrimidine tract-binding protein 1; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; PTBP1; RNA-binding protein

Database Links

UniProt ID: P26599-1

Entrez-Gene Id: 5725

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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PTBP1 (E5O2S) Rabbit Monoclonal Antibody #72669