R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PSPC1 (E7L8B) Rabbit mAb #65992
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PSPC1 (E7L8B) Rabbit mAb recognizes endogenous levels of total PSPC1 protein. Faint bands running around 30 kDa, 80 kDa, and 100 kDa were observed. We are unclear of the identity of these bands.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PSPC1 protein.
Background
PSPC1 (paraspeckle component 1) was one of the first proteins found to be enriched in subnuclear bodies that are adjacent to but distinct from nuclear speckles, and subsequently named paraspeckles (1). PSPC1 belongs to the Drosophila melanogaster behavior protein family comprised of human splicing (DBHS) proteins, including SFPQ (splicing factor proline-glutamine rich ) and NONO (non-POU domain-containing octamer-binding protein) which are also enriched in paraspeckles (2). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, transcript localization, splicing, and DNA damage repair (3). Important to disease, DBHS proteins bind the long noncoding RNA NEAT1 which is found to be enriched in paraspeckles. Aberrant accumulation of NEAT1 transcripts in paraspeckles has been identified in multiple tumor types, leading to a proposal that the long coding RNA, via the process of sponging, disrupts the ratio of miRNAs to target proteins, resulting in altered protein expression profiles that promote cancer (4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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