Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PSD95 (D27E11) XP® Feline Chimeric mAb #90708
Filter:
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Feline Chimera IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as PSD95 (D27E11) XP® Rabbit mAb #3450.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PSD95 (D27E11) XP® Feline Chimeric mAb recognizes endogenous levels of total PSD95 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from PSD95 (D27E11) XP® Rabbit mAb #3450 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, PSD95 (D27E11) XP® Rabbit mAb #3450, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln53 of human PSD95 protein.
The parent antibody, PSD95 (D27E11) XP® Rabbit mAb #3450, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln53 of human PSD95 protein.
Background
Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience-dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).
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For Research Use Only. Not for Use in Diagnostic Procedures.
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