Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
POFUT1 (F5I5W) Rabbit mAb #66762
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
POFUT1 (F5I5W) Rabbit mAb recognizes endogenous levels of total POFUT1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human POFUT1 protein.
Background
Notch signaling is essential for lymphocyte development and myelopoiesis (1,2,4,6). Notch receptors are modified by O-fucosylation catalyzed by the enzyme protein O-fucosyltransferase 1 (POFUT1) (1,2,4). POFUT1 fucosylates epidermal growth factor (EGF)-like domains found at the cell surface and in secreted glycoproteins, including Notch and its ligands. POFUT1 catalyzes the reaction attaching fucose via O-glycosidic linkage to conserved serine or threonine residues in EGF-like domains (2,7). This particular Notch modification is critical for development and cell fate; POFUT1 deficiency leads to aberrant signaling and human disease (3,5,8,9). In addition, mouse embryonic stem cells lacking POFUT1 have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or display proper Notch signaling (4,6).
- Wang, Y. et al. (2001) J Biol Chem 276, 40338-45.
- McMillan, B.J. et al. (2017) Glycobiology 27, 777-786.
- Du, Y. et al. (2018) Cell Death Dis 9, 995.
- Stahl, M. et al. (2008) J Biol Chem 283, 13638-51.
- Barlak, N. et al. (2023) Cell Biol Int 47, 1950-1963.
- Yao, D. et al. (2011) Blood 117, 5652-62.
- Li, Z. et al. (2017) Nat Chem Biol 13, 757-763.
- Zhang, C. et al. (2019) Cell Death Dis 10, 477.
- Chabanais, J. et al. (2018) Cancers (Basel) 10, 411. doi: 10.3390/cancers10110411.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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