Phospho-Lamin B1 (Ser210) Antibody #17772
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
| MW (kDa) | 68 |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Lamin B1 (Ser210) Antibody recognizes endogenous levels of lamin B1 protein only when phosphorylated at Ser210.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser210 of human lamin B1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
Phosphorylation of lamin B1 at Ser210 in the rod domain by the combined activities of the autophagy kinase ULK1 and the mitotic kinases Aurora A and PLK1 contributes to mitotic spindle maintenance. For more information, visit Biorxiv at biorxiv.org/content/10.1101/2025.04.25.650690v1.
Phosphorylation of lamin B1 at Ser210 in the rod domain by the combined activities of the autophagy kinase ULK1 and the mitotic kinases Aurora A and PLK1 contributes to mitotic spindle maintenance. For more information, visit Biorxiv at biorxiv.org/content/10.1101/2025.04.25.650690v1.
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
- Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
- Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
- Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
- Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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