Phospho-DARPP-32 (Thr34) Antibody #2304
Inquiry Info. # 2304
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Supporting Data
| REACTIVITY | |
| SENSITIVITY | Endogenous |
| MW (kDa) | 32 |
| SOURCE | Rabbit |
Product Information
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-DARPP-32 (Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34. The antibody does not cross-react with DARPP-32 phosphorylated at Thr75. This antibody may also detect PP-1 inhibitor.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32. Antibodies are purified by protein A and peptide affinity chromatography.
Background
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).
Alternate Names
darpp-32; darpp32
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