Phospho-Aurora B/AIM1(Thr232) Antibody #3095
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Supporting Data
| REACTIVITY | |
| SENSITIVITY | |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Product Information
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-Aurora B/AIM1 Antibody detects endogenous levels of Aurora B/AIM1 only when phosphorylated at threonine 232.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr232 of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
Aurora B autophosphorylation at Thr232 has been shown to be an important in allowing Aurora B activation and proper cytokinesis (7).
Aurora B autophosphorylation at Thr232 has been shown to be an important in allowing Aurora B activation and proper cytokinesis (7).
- Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
- Zhou, H. et al. (1998) Nat Genet 20, 189-93.
- Sen, S. et al. (1997) Oncogene 14, 2195-200.
- Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
- Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
- Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
- Yasui, Y. et al. (2004) J Biol Chem 279, 12997-3003.
Alternate Names
AIK2; AIM-1; AIM1; ARK2; AurB; IPL1; STK12
限制使用
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