Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Peripherin (F3J1R) Rabbit mAb #85624
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Peripherin (F3J1R) Rabbit mAb recognizes endogenous levels of total peripherin protein. This antibody detects a peripherin dimer at 140 kDa and may also detect other peripherin isoforms.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse peripherin protein.
Background
The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Peripherin is a class-III intermediate filament protein selectively expressed in peripheral nervous system (PNS) neurons (1). Several peripherin isoforms exist through alternative splicing, with the dominant isoform occurring at 58 kDa and minor isoforms occurring at 48, 45, 32, and 25 kDa (2,3). Additionally, an aggregate-forming 28 kDa splice isoform of peripherin has been shown to be upregulated in Amyotrophic Lateral Sclerosis, suggesting a role in disease pathology (3). Functionally, peripherin plays an important role in neurite outgrowth, axonal transport, and myelination; it also interacts with proteins responsible for vesicular trafficking (4). Because of its nearly exclusive expression in PNS neurons, it may be a promising blood biomarker of PNS axonal damage (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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