R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PBEF/NAMPT (D1K6D) Rabbit mAb #61122
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:1600 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PBEF/NAMPT (D1K6D) Rabbit mAb recognizes endogenous levels of total PBEF/NAMPT protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly122 of human PBEF/NAMPT protein.
Background
Nicotinamide phosphoribosyltransferase (NAMPT; also known as Pre-B cell-enhancing factor PBEF) catalyzes the synthesis of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosylpyrophosphate (PRPP), the rate-limiting step in the NAD biosynthesis pathway starting from nicotinamide (1,2). NAD biosynthesis mediated by NAMPT plays a critical role in glucose-stimulated insulin secretion in pancreatic beta cells (3). Both NAMPT inhibitors and activators have been sought for clinical applications (4,5). NAMPT has intra- and extracellular forms (iNAMPT and eNAMPT), and deacetylation of iNAMPT by SIRT1 promotes eNAMPT secretion through a nonclassical secretory pathway (3,6). eNAMPT, independent of its enzymatic activity, can induce epithelial-to-mesenchymal transition in mammary epithelial cells and promote monocyte differentiation into a tumor-supporting M2 macrophage (7,8).
- Imai, S. (2009) Curr Pharm Des 15, 20-8.
- Samal, B. et al. (1994) Mol Cell Biol 14, 1431-7.
- Revollo, J.R. et al. (2007) Cell Metab 6, 363-75.
- Montecucco, F. et al. (2013) Curr Drug Targets 14, 637-43.
- Wang, G. et al. (2014) Cell 158, 1324-34.
- Yoon, M.J. et al. (2015) Cell Metab 21, 706-17.
- Soncini, D. et al. (2014) J Biol Chem 289, 34189-204.
- Audrito, V. et al. (2015) Blood 125, 111-23.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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