R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PAXX (D6X7X) Rabbit mAb #92448
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PAXX (D6X7X) Rabbit mAb recognizes endogenous levels of total PAXX protein.
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro195 of human PAXX protein.
Background
DNA double-strand breaks (DSBs) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. In mammalian cells, the most prominent mechanism by which cells deal with DSBs is known as NHEJ (non-homologous end joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1).
PAXX, (Paralog of XRCC4 and XLF, also known as C9orf142 or XLS), is a more recently identified component of the NHEJ machinery whose crystal structure resembles that of XRCC4 (2). PAXX directly interacts with Ku, and promotes accumulation of Ku at DSBs (2,3). Depletion of PAXX impairs cellular DSB repair (2-4,5). Paxx -/- mice develop normally with mild radiosensitivity, but a Paxx/Xlf double knockout is embryonic lethal in mice, indicating synthetic lethality between Paxx and Xlf (6). Paxx/Xlf double knockout have increased apopotosis in post-mitotic motor neurons, as well as impaired development of the adaptive immune system (7).
PAXX, (Paralog of XRCC4 and XLF, also known as C9orf142 or XLS), is a more recently identified component of the NHEJ machinery whose crystal structure resembles that of XRCC4 (2). PAXX directly interacts with Ku, and promotes accumulation of Ku at DSBs (2,3). Depletion of PAXX impairs cellular DSB repair (2-4,5). Paxx -/- mice develop normally with mild radiosensitivity, but a Paxx/Xlf double knockout is embryonic lethal in mice, indicating synthetic lethality between Paxx and Xlf (6). Paxx/Xlf double knockout have increased apopotosis in post-mitotic motor neurons, as well as impaired development of the adaptive immune system (7).
- Tsai, C.J. et al. (2007) Proc Natl Acad Sci U S A 104, 7851-6.
- Ochi, T. et al. (2015) Science 347, 185-188.
- Liu, X. et al. (2017) Nat Commun 8, 13816.
- Craxton, A. et al. (2015) Cell Death Differ 22, 890-7.
- Xing, M. et al. (2015) Nat Commun 6, 6233.
- Balmus, G. et al. (2016) Genes Dev 30, 2152-2157.
- Abramowski, V. et al. (2017) Cell Death Differ, 444-452.
限制使用
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