R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Oxytocin/Neurophysin I (F3X6H) Rabbit mAb #93766
Filter:
- IHC
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Oxytocin/Neurophysin I (F3X6H) Rabbit mAb recognizes endogenous levels of total oxytocin/neurophysin I protein. Non-specific staining was observed in human skeletal myocytes by immunohistochemistry.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of human oxytocin/neurophysin I protein.
Background
Encoded by the OXT gene, the neuropeptide oxytocin and its carrier protein neurophysin I are synthesized primarily in magnocellular neurons of the hypothalamus. Once synthesized, oxytocin is either secreted in the brain or transported to the neurohypophysis via neurosecretory vesicles, where it is stored and released into the bloodstream (1). Oxytocin acts by binding its lone receptor, OXTR, which in turn binds to Gα(i) or Gα(q) proteins, activating a number of signaling cascades, including mitogen-activated protein kinase (MAPK), protein kinase C (PKC), phospholipase C (PLC), and Ca2+/calmodulin-dependent protein kinase (CaMK) pathways (1,2). Oxytocin was first described for its important role in parturition and lactation and has since been associated with a number of social behaviors (1,3). Cyclic ADP-ribose hydrolase 1 (CD38) is a key mediator of oxytocin secretion. Genetic polymorphisms related to the oxytocin system, including in the CD38 and OXTR genes, have been linked to autism spectrum disorder (ASD) (4,5). Oxytocin also has roles in cognitive function and has been proposed as a therapeutic target for Alzheimer’s disease (AD) (2).
- Jurek, B. and Neumann, I.D. (2018) Physiol Rev 98, 1805-1908.
- Takahashi, J. et al. (2023) Cells 12, 2426. doi: 10.3390/cells12202426.
- Ross, H.E. and Young, L.J. (2009) Front Neuroendocrinol 30, 534-547.
- Baribeau, D.A. and Anagnostou, E. (2015) Front Neurosci 9, 335.
- Zhang, R. et al. (2017) Neurosci Bull 33, 238-246.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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