Numb Antibody #2733
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Inquiry Info. # 2733
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 72, 74 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Numb Antibody detects endogenous levels of total Numb protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Numb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9).
- Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
- Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
- Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
- Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
- Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
- McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
- Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
- Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
- Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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