Name: mTOR (L27D4) Mouse mAb
Price: 336.00 USD
Availability: InStock

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WB

Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	289
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:50 - 1:250

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

mTOR (L27D4) Mouse mAb detects endogenous levels of total mTOR protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human mTOR protein.

Background

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

Alternate Names

FK506 binding protein 12-rapamycin associated protein 1; FRAP1; Mammalian target of rapamycin; mTOR; rapamycin; Rapamycin target protein (RAPT1); RAPT; RAPT1; TOR

Database Links

UniProt ID: P42345

Entrez-Gene Id: 2475

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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mTOR (L27D4) Mouse mAb #4517