Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MRP3/ABCC3 (D8V8J) Rabbit mAb #39909
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160-220 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MRP3/ABCC3 (D8V8J) Rabbit mAb recognizes endogenous levels of total MRP3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Gly870 of human MRP3 protein.
Background
MRP3/ABCC3 belongs to the super family of ATP-binding cassette (ABC) transporters. It is a member of the MRP subfamily that is expressed in various organs including liver, gallbladder, small intestine, colon, kidney, and adrenal gland (1-3). MRP3 is involved in multi-drug resistance (1). It facilitates the efflux of organic anions including monoanionic bile acid and anti-cancer reagents such as etoposide and paclitaxel from liver and small intestine into blood (4-7). Expression of MRP3 is increased in the cholestatic human and rat liver, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts (2,8). MRP3 expression is also upregulated in people with Dubin-Johnson Syndrome (DJS) who lack functional MRP2 in the liver, which implicates the compensatory role of MRP3 in the absence of functional MRP2 (4).
Elevated expression of MRP3 has been detected in various cancer types such as hepatocellular carcinomas, primary ovarian cancer, and adult acute lymphoblastic leukemia (ALL) (9-11). Overexpression of MRP3 was reported to be a prognostic factor in ALL and adult acute myeloid leukemia (AML) (11,12).
Elevated expression of MRP3 has been detected in various cancer types such as hepatocellular carcinomas, primary ovarian cancer, and adult acute lymphoblastic leukemia (ALL) (9-11). Overexpression of MRP3 was reported to be a prognostic factor in ALL and adult acute myeloid leukemia (AML) (11,12).
- Uchiumi, T. et al. (1998) Biochem Biophys Res Commun 252, 103-10.
- Scheffer, G.L. et al. (2002) Lab Invest 82, 193-201.
- Kiuchi, Y. et al. (1998) FEBS Lett 433, 149-52.
- König, J. et al. (1999) Hepatology 29, 1156-63.
- Zelcer, N. et al. (2001) J Biol Chem 276, 46400-7.
- Belinsky, M.G. et al. (2005) Mol Pharmacol 68, 160-8.
- Dickerson, W.G. (1992) Dent Today 11, 54-5.
- Soroka, C.J. et al. (2001) Hepatology 33, 783-91.
- Nies, A.T. et al. (2001) Int J Cancer 94, 492-9.
- Ohishi, Y. et al. (2002) Clin Cancer Res 8, 3767-75.
- Plasschaert, S.L. et al. (2005) Clin Cancer Res 11, 8661-8.
- Benderra, Z. et al. (2005) Clin Cancer Res 11, 7764-72.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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