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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MLXIP/MondoA (F8A8L) Rabbit mAb #72133

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MLXIP/MondoA (F8A8L) Rabbit mAb recognizes endogenous levels of total MLXIP/MondoA protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLXIP/MondoA protein.

    Background

    MLX-interacting protein (MLXIP), also known as the transcriptional activator MondoA, forms a heterodimer with MLX to bind to DNA, activating transcription of genes involved in glycolysis (1,2). Studies show that MLXIP/MondoA functions as a metabolic stress sensor in common B-cell acute lymphoblastic leukemia (B-ALL) cells by downregulating pyruvate dehydrogenase activity, inhibiting oxidative phosphorylation. Furthermore, MLXIP/MondoA overexpression correlates with poor survival rates in pediatric common acute lymphoblastic leukemia, suggesting that MLXIP/MondoA contributes to B-ALL malignancy by mediating adaptive responses to metabolic stress (3). In addition, studies show that MLXIP/MondoA is a regulator of cellular senescence. It delays cellular senescence by maintaining mitochondrial homeostasis and activating autophagy (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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