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50261
MCU (D2Z3B) Rabbit mAb (BSA and Azide Free)
一抗
单克隆抗体
R
Recombinant

MCU (D2Z3B) Rabbit mAb (BSA and Azide Free) #50261

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Filter:
  1. WB
  2. IF
Western blot analysis of extracts from various cell lines using MCU (D2Z3B) Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of fixed frozen mouse retina, labeled with MCU (D2Z3B) Rabbit mAb #14997 (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red) and ProLong® Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of fixed frozen mouse colon, labeled with MCU (D2Z3B) Rabbit mAb #14997 (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red) and ProLong® Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HCT 116 cells, labeled with MCU (D2Z3B) Rabbit mAb #14997 (left, green) and co-labeled with DyLight 650 Phalloidin #12956 (right, red), and DAPI #4083 (right, blue). Data were generated using the standard formulation of this product.
To Purchase # 50261SF
Cat. # Size Price Inventory
50261SF
100 µg

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 30
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #14997. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #14997

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

MCU (D2Z3B) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total MCU protein. The antibody non-specifically labels the nucleus in fixed frozen mouse brain by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MCU protein.

Background

The mitochondrial calcium uniporter (MCU) is an inner membrane transport protein that is essential for the regulation of calcium uptake and the maintenance of mitochondrial calcium homeostasis (1). MCU is responsible for the rapid transport of calcium ions across the inner membrane and into the mitochondrial matrix, taking advantage of the membrane potential generated by the electron transport chain. Subsequent release of calcium from the matrix through antiporter proteins regulates a number of biological processes, including signal transduction, bioenergetics, and cell death and survival (2,3). The MCU protein contains a pair of transmembrane domains with both carboxy- and amino-terminal ends within the mitochondrial matrix (3). The surrounding uniporter complex contains a number of proteins that regulate calcium ion transport, including the mitochondrial calcium uniporter regulator 1 (MCUR1), mitochondrial calcium uptake proteins 1 and 2 (MICU1, MICU2), and the essential MCU regulator EMRE (3). MICU1 stabilizes the closed state of the transport complex and preserves the normal mitochondrial calcium concentration below the equilibrium level during resting conditions (4). The MCUR1 protein is an essential regulator of calcium uptake, with decreased ion transport in cells with reduced MCUR1 expression (2). EMRE is also essential for MCU transport function where it functions as bridge between MCU uniporter activity and the calcium-sensing MICU1/2 proteins (5).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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