Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LPCAT3 (E5G9P) Rabbit mAb #72964
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 36 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LPCAT3 (E5G9P) Rabbit mAb recognizes endogenous levels of total LPCAT3 protein. This antibody may detect nonspecific bands at 90 and 160 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human LPCAT3 protein.
Background
LPCAT3 (lysophosphatidylcholine acyltransferase 3), also called MBOAT5, is part of the LPCAT family that plays an important role in lipid metabolism (1,2). Its function is to catalyze the incorporation of fatty acids into phospholipids, lysophosphatidylcholine (lysoPC), lysophosphatidylserine (lysoPS), and lysophosphatidylethanolamine (lysoPE), to produce various fatty acid containing forms of PC, PS, and PE. It shows a preference for lysoPC and incorporation of linoleoyl and arachidonoyl chains as fatty acid donors (3,4). LPCAT3 is localized to the endoplasmic reticulum (ER) and is primarily expressed in metabolic tissues, including the liver, pancreas, and adipose tissue (2). It is a direct transcriptional target of LXR, PPARα, and PPARγ, which are lipid activated nuclear receptors that play important roles in regulating lipid homeostasis (2,5-7). Elevated expression of LPCAT3 is associated with the iron-dependent cell death process of ferroptosis that is characterized by an increase in lipid peroxidation (8). LPCAT3 inhibitors have shown a partial protection from ferroptosis (9). Dysregulation of LPCAT3 can have an important role in the development of diseases, including atherosclerosis, diabetes, intestinal tumors, and nonalcoholic steatohepatitis (NASH) (10,11). LPCAT3 is significantly upregulated in many cancers and is associated with prognosis (12).
- Matsuda, S. et al. (2008) Genes Cells 13, 879-88.
- Zhao, Y. et al. (2008) J Biol Chem 283, 8258-65.
- Kazachkov, M. et al. (2008) Lipids 43, 895-902.
- Gijón, M.A. et al. (2008) J Biol Chem 283, 30235-45.
- Rong, X. et al. (2013) Cell Metab 18, 685-97.
- Demeure, O. et al. (2011) Gene 470, 7-11.
- Singh, A.B. and Liu, J. (2017) J Biol Chem 292, 884-897.
- Lee, J.Y. et al. (2021) Biology (Basel) 10, 184. doi: 10.3390/biology10030184.
- Reed, A. et al. (2022) ACS Chem Biol 17, 1607-1618.
- Shao, G. et al. (2022) J Cancer 13, 2430-2439.
- Wang, B. and Tontonoz, P. (2019) Annu Rev Physiol 81, 165-188.
- Ke, P. et al. (2022) Transl Cancer Res 11, 3491-3505.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.