Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Layilin (E9U6M) Rabbit mAb #42746
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43, 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Layilin (E9U6M) Rabbit mAb recognizes endogenous levels of total Layilin protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Layilin protein.
Background
Layilin (LAYN) is a single-pass type I integral membrane protein widely expressed by different cell types and tissues (1). LAYN is a hyaluronan receptor that directly interacts with several cytoskeletal-membrane linker molecules, including talin, merlin, and radixin (1-3). In both tumor and immune cell types, LAYN plays an important role in cell adhesion and motility, promoting cell spreading, migration, and infiltration (1,4-7). LAYN is involved in the tumor necrosis factor-α induced epithelial-mesenchymal transition of renal tubular epithelial cells (8), and also plays a critical role in HA35-induced intestinal epithelial tight junctions in inflammatory bowel disease (9). Lineage-specific deletion of LAYN on murine CD8+ T cells reduced their accumulation in tumors and increased tumor growth in vivo (10). Multiple isoforms have been identified.
- Borowsky, M.L. and Hynes, R.O. (1998) J Cell Biol 143, 429-42.
- Bono, P. et al. (2001) Mol Biol Cell 12, 891-900.
- Bono, P. et al. (2005) Exp Cell Res 308, 177-87.
- Chen, Z. et al. (2008) Biotechnol Appl Biochem 50, 89-96.
- Kaji, T. et al. (2019) Brain Res 1719, 140-147.
- Pan, J.H. et al. (2019) Front Immunol 10, 6.
- Zheng, C. et al. (2017) Cell 169, 1342-1356.e16.
- Adachi, T. et al. (2015) Biochem Biophys Res Commun 467, 63-9.
- Kim, Y. et al. (2018) Matrix Biol 66, 93-109.
- Mahuron, K.M. et al. (2020) J Exp Med 217, e20192080. doi: 10.1084/jem.20192080.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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