R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Kindlin-3 (D8I7V) Rabbit mAb #10459
Filter:
- WB
- IP
- IHC
- F
Supporting Data
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 75 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:400 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Kindlin-3 (D8I7V) Rabbit mAb recognizes endogenous levels of total Kindlin-3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg648 of human Kindlin-3 protein.
Background
The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3).
Kindlin-3 (FERMT3 or URP2) is a member of the kindlin family of focal adhesion proteins, which plays an essential role in integrin activation and in various immune functions (3,4). Kindlin-3 is mainly expressed in the hematopoietic system (5). Kindlin-3, together with talin, binds to the cytoplasmic tail of β-integrin and activates integrin-mediated signaling cascades (6-8). Research studies identified mutations of Kindlin-3 that cause a rare hereditary human disease, leukocyte-adhesion deficiency (LAD) type III (9,10).
Kindlin-3 (FERMT3 or URP2) is a member of the kindlin family of focal adhesion proteins, which plays an essential role in integrin activation and in various immune functions (3,4). Kindlin-3 is mainly expressed in the hematopoietic system (5). Kindlin-3, together with talin, binds to the cytoplasmic tail of β-integrin and activates integrin-mediated signaling cascades (6-8). Research studies identified mutations of Kindlin-3 that cause a rare hereditary human disease, leukocyte-adhesion deficiency (LAD) type III (9,10).
- Ye, F. and Petrich, B.G. (2011) Curr Opin Hematol 18, 356-60.
- Meves, A. et al. (2009) Trends Cell Biol 19, 504-13.
- Karaköse, E. et al. (2010) J Cell Sci 123, 2353-6.
- Fagerholm, S.C. et al. (2014) Am J Clin Exp Immunol 3, 37-42.
- Pasini, E.M. et al. (2006) Blood 108, 791-801.
- Tadokoro, S. et al. (2003) Science 302, 103-6.
- Wegener, K.L. et al. (2007) Cell 128, 171-82.
- Moser, M. et al. (2009) Science 324, 895-9.
- Malinin, N.L. et al. (2009) Nat Med 15, 313-8.
- Svensson, L. et al. (2009) Nat Med 15, 306-12.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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