R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Intersectin-2 (E5J9F) Rabbit mAb #43069
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200, 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Intersectin-2 (E5J9F) Rabbit mAb recognizes endogenous levels of total intersectin-2 protein. This antibody reacts with intersectin-2L and intersectin-2S protein isoforms but does not cross-react with the corresponding isoforms of intersectin-1 protein. This antibody cross-reacts with proteins of unknown identity between 80 kDa and 120 kDa in some rat cell extracts.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys335 of human intersectin-2 protein.
Background
Intersectin-2 is a ubiquitously expressed, modular adaptor protein that participates in clathrin-mediated endocytosis through interaction with multiple proteins involved in trafficking of proteins through the endocytic pathway. Like its paralog, intersectin-1, there are two major isoforms of mammalian intersectin-2. Intersectin-2L and intersectin-2S are generated through alternative splicing of mRNA transcripts. The structure of both isoforms is characterized by two N-terminal EH domains, a central coiled-coil domain, followed by five tandem SH3 domains. The longer isoform, intersection-2L, contains an extended C-terminus with a Rho-GEF domain that controls the activity of the Cdc42 GTPase, a PH domain, and a calcium binding C2 domain (1-3). Research studies have shown that intersectin-2 plays a role in T cell receptor (TCR) signaling by promoting endocytosis of the TCR following its activation (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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