R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HHLA2/B7-H7 (E1U6X) Rabbit mAb #52200
Filter:
- WB
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 70-80 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HHLA2/B7-H7 (E1U6X) Rabbit mAb recognizes endogenous levels of total HHLA2/B7-H7 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn101 of human HHLA2/B7-H7 protein.
Background
HERV-H LTR-associating protein 2 (HHLA2, with alternative names of B7-H5 and B7-H7) is a member of the B7 immunoglobulin superfamily (1). HHLA2 protein is constitutively expressed on the surface of human monocytes and is induced on B cells after stimulation with LPS and IFN-γ (1,2). Through interaction with TMIGD2, which is constitutively expressed on all naïve T cells and the majority of natural killer cells, but not on T regulatory cells or B cells, HHLA2 co-stimulates T cells in the context of TCR-mediated activation, enhancing T cell proliferation and cytokine production via an AKT-dependent signaling cascade (2). Contrary to this, HHLA2 has also been shown to inhibit T cell proliferation and cytokine production, suggesting a secondary receptor for HHLA2 that is expressed on activated T cells with co-inhibitory functions (3). Moreover, HHLA2 has been shown to be highly expressed in various types of cancer, and is associated with a poor prognosis (4-10). Further understanding the immunologic functions of the HHLA2 pathway will guide the selection of agents used for cancer immunotherapy, autoimmune disorders, infection, and transplant rejection.
- Mager, D.L. et al. (1999) Genomics 59, 255-63.
- Zhu, Y. et al. (2013) Nat Commun 4, 2043.
- Zhao, R. et al. (2013) Proc Natl Acad Sci U S A 110, 9879-84.
- Janakiram, M. et al. (2015) Clin Cancer Res 21, 2359-66.
- Koirala, P. et al. (2016) Sci Rep 6, 31154.
- Cheng, H. et al. (2017) Clin Cancer Res 23, 825-32.
- Cheng, H. et al. (2018) Clin Cancer Res 24, 1954-64.
- Zhu, Z. and Dong, W. (2018) Onco Targets Ther 11, 1563-70.
- Shimonosono, M. et al. (2018) Oncotarget 9, 22069-78.
- Chen, D. et al. (2018) J Med Genet 56, 43-49.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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